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- 组织来源：
ascites
- 细胞形态：
淋巴样
- 相关疾病：
淋巴瘤
- 运输方式：
冻存运输
- ATCC Number：
HTB-62™
- 生长状态：
悬浮生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 年限：
7 years
- 细胞类型：
B淋巴细胞
- 库存：
大量
| Designations: | P3HR-1 | ||
| Depositors: | G Trempe, LJ Old | ||
| Biosafety Level: | 2 [Cells Contain Herpesvirus ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Tissue: ascites Disease: Burkitt's lymphoma Cell Type: B lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 10,11 D5S818: 12 D7S820: 8,10 THO1: 7,9 TPOX: 6,8 vWA: 15,19 |
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| Isoenzymes: | ES-D, 1 G6PD, B GLO-I, 1 Me-2, 2 PGM1, 1 |
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| Age: | 7 years | ||
| Gender: | male | ||
| Ethnicity: | Black, African | ||
| Comments: | The P3HR-1 line is a clonally derived subline of Jiyoye (ATCC CCL-87 ) which yielded up to 50-85% immunofluorescent positive cells when stained with selected human sera. Herpes type virus particles (Epstein-Barr virus) were observed in as many as 78% of the cells examined with the electron microscope and a good correlation between presence of viral particles and immunofluorescence was obtained. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium, 80%; heat-inactivated fetal bovine serum, 20% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | derivative:ATCC CCL-87 | ||
| References: | 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722 22760: . . Sci. Rep. Res. Inst. Tohoku Univ. Ser. C Med. 16: 97-101, 1969. 22816: Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447 22878: Hinuma Y, et al. Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol. 1: 1045-1051, 1967. PubMed: 4912237 23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014 |
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文献和实验P3实验室是生物安全防护三级实验室。生物安全防护实验室根据微生物及其毒素的危害程度不同，分为4级，一级最低，四级最高。一级实验室一般适用于对健康成年人无致病作用的微生物；二级适用于对人和环境有中等潜在危害的微生物；三级适用于主要通过呼吸途径使人传染上严重的甚至是致死疾病的致病微生物或其毒素；四级适用于对人体具有高度的危险性，通过汽溶胶途径传播或传播途径不明、目前尚无有效疫苗或治疗方法的致病微生物或其毒素。P3实验室分为细胞水平的和动物水平的两类，动物水平的又分为小动物水平和大动物水平。我国
，禁止与进行实验无关之人员进入。 14）实验进行中，要在实验室及实验区域之入口处，标示「P3级实验室」，并挂上「P3级实验进行中」的标示。而且保存重组体之冰箱及冷冻库，也要做同样的标示。 15）实验室需经常整理，保持清洁，不要放置与实验无关的物品。 16）安全操作装置内之HEPA过滤器，在更换前，定期检查及实验内容变更时，需密封安全操作装置，每立方公尺用10公克甲醛熏蒸1小时，去除污染。 17）在此级实验室内，如欲同时进行级数较低之实验，需按照本级之规定实施。 18）遵守计划主持人所规定
●负压密闭实验室处于一个完全密闭环境中，环境气压要保持在40Pa～60Pa的负压状态，进入的空气不可能倒流。●空气过滤进入实验室的空气都是经过完全过滤的；排出的空气也必须经过消毒、过滤处理以保证洁净。●严格消毒进入P3实验室的人员必须遵守严格消毒程序规定：工作人员在进入实验室前必须消毒，必须穿着隔离服和戴眼罩、口罩、手套等防护用具。人员必须进行消毒处理、换装后才能离开。实验室产生的废物及工作服等一律不能带出，需要经过物流通道先消毒，再通过传递窗进入高压灭菌锅，在消毒合格后才能进行下一步的垃圾
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