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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 生长状态：
贴壁生长
- 年限：
46 years
- 相关疾病：
肿瘤
- ATCC Number：
HTB-134™
- 细胞形态：
上皮样
- 器官来源：
胰腺
| Designations: | Hs 766T | ||
| Depositors: | RB Owens | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: pancreas Disease: carcinoma Derived from metastatic site: lymph node |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 8,11 D16S539: 9,12 D5S818: 11 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 18 |
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| Cytogenetic Analysis: | (P11) hyperdiploid with mode = 60; banded markers; lacking Y chromosomes | ||
| Isoenzymes: | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1-2 PGM3, 1 |
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| Age: | 46 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The Hs 766T line was isolated by R. Owens et al. at the Naval Biosciences Laboratory in 1973 from a pancreatic carcinoma metastatic to a lymph node. The epithelial nature of the cell strain selected was confirmed by electron microscopy as microvilli and desmosomes were observed. Tests conducted at an early passage indicated that the cells exhibited many properties observed only in tumor lines. The karyotype was abnormal and the cells formed colonies in Methocel and on contact inhibited monolayers. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258 23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 26328: . Prevention and detection of cancer. vol. 2New York: Marcel Dekker; 1978. |
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Hs 766T
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