- 询价
- 2025年09月05日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 运输方式：
冻存运输
- 细胞形态：
成纤维样
- 相关疾病：
神经胶质瘤
- ATCC Number：
HTB-138™
- 年限：
76 years
- 生长状态：
贴壁生长
- 器官来源：
大脑
| Designations: | Hs 683 | ||
| Depositors: | RB Owens | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
||
| Source: | Organ: brain Disease: glioma |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | No | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,13 D13S317: 8,12 D16S539: 9,10 D5S818: 11,12 D7S820: 11 THO1: 6,8 TPOX: 8,11 vWA: 18,20 |
||
| Cytogenetic Analysis: | modal number = 82; range = 74 to 87. This is a hypertriploid human cell line with the modal chromosome number of 82 occurring in 16% of cells, with the distribution skewed toward the lower number range. Higher ploidies occurred in 3.0% of cells. Most cells had 4-6 marker chromosomes, but only del(3) (q12.2q21) occurred frequently in most cells, and der(9)t(9;?) (q34.2;?), tdic(1;4) (q21;q35), and a minute acrocentric occurred in more than two cells. N7 occurred in 4-6 copies per cell. Two X and one or two Y chromosomes were present in most cells. |
||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1-2 |
||
| Age: | 76 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Hs 683 was isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian. Microvilli but no desmosomes were observed. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: Twice per week |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| References: | 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 23221: Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 32550: Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
猴 B病毒 (HS) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定猴血清，血浆及相关液体样本中 B病毒(HS) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 猴 B病毒 (HS) 水平。用纯化的 猴 B病毒 (HS) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 B病毒 (HS) ， 再与HRP 标记的 B 病毒 (HS) 抗体结合，形成
人 高敏 C 反应蛋白（ hs-CRP ） 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 高敏C 反应蛋白（hs-CRP ） 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 人高敏 C 反应蛋白（ hs-CRP ） 水平。用纯化的 人 高敏 C 反应蛋白（ hs-CRP ） 抗体包被微孔板，制成固相抗体，往包
HOZAN L系列 L-678 LED放大镜 HOZAN HS系列 HS-26-230 温调试电烙铁 HOZAN HS系列 HS-26-100V 温调试电烙铁 HOZAN F系列 F-126 静电脚腕带 HOZAN DT系列 D-745 测电笔 HOZAN F系列 F-64-S 手套 HOZAN F系列 F-64-M 手套 HOZAN F系列 F-64-L 手套 HOZAN Z系列 Z-275 清洗剂 HOZAN Z系列
技术资料暂无技术资料 索取技术资料
Hs 683
询价
