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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 运输方式：
冻存运输
- 细胞形态：
成纤维样
- 相关疾病：
神经胶质瘤
- ATCC Number：
HTB-138™
- 年限：
76 years
- 生长状态：
贴壁生长
- 器官来源：
大脑
| Designations: | Hs 683 | ||
| Depositors: | RB Owens | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: brain Disease: glioma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | No | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,13 D13S317: 8,12 D16S539: 9,10 D5S818: 11,12 D7S820: 11 THO1: 6,8 TPOX: 8,11 vWA: 18,20 |
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| Cytogenetic Analysis: | modal number = 82; range = 74 to 87. This is a hypertriploid human cell line with the modal chromosome number of 82 occurring in 16% of cells, with the distribution skewed toward the lower number range. Higher ploidies occurred in 3.0% of cells. Most cells had 4-6 marker chromosomes, but only del(3) (q12.2q21) occurred frequently in most cells, and der(9)t(9;?) (q34.2;?), tdic(1;4) (q21;q35), and a minute acrocentric occurred in more than two cells. N7 occurred in 4-6 copies per cell. Two X and one or two Y chromosomes were present in most cells. |
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| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1-2 |
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| Age: | 76 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Hs 683 was isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian. Microvilli but no desmosomes were observed. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| References: | 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 23221: Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 32550: Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406 |
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Hs 683
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