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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 器官来源：
胰腺
- 库存：
大量
- 生长状态：
贴壁生长
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1918™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 年限：
26 years
| Designations: | CFPAC-1 | ||
| Depositors: | RA Schoumacher | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: pancreas Disease: ductal adenocarcinoma; cystic fibrosis Derived from metastatic site: liver metastasis |
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| Cellular Products: | carcinoembryonic antigen (CEA), 9 ng/ml; pancreatic oncofetal antigen (POA), 28 ng/ml; adenocarcinoma associated antigen (ACAA), 5000 ng/ml; CA 19-9 antigen, 12000 units/ml; epithelial keratins | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | CA19-9 antigen, 12000 units/ml | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 9,11 D5S818: 10,11 D7S820: 8,10 THO1: 8 TPOX: 8 vWA: 17 |
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| Cytogenetic Analysis: | hyperdiploid; modal number = 73; range = 65 to 75; several translocations were evident; two to three apparently normal copies of number 7 (to which the cystic fibrosis gene has been mapped) were present in every metaphase examined | ||
| Age: | 26 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line was derived from a ductal adenocarcinoma (liver metastasis) from a patient with cystic fibrosis. The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene (cystic fibrosis transmembrane regulator, CFTR). CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca++ ionophores with increase Cl- efflux. The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508. CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 32 hrs | ||
| References: | 1785: Schoumacher RA, et al. A cystic fibrosis pancreatic adenocarcinoma cell line. Proc. Natl. Acad. Sci. USA 87: 4012-4016, 1990. PubMed: 1692630 22227: McIntosh JC, et al. Pancreatic adenocarcinoma in a patient with cystic fibrosis. Am. J. Med. 85: 592, 1988. PubMed: 3177424 |
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