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- 2026年01月26日
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- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- 运输方式：
冻存运输
- 相关疾病：
正常
- ATCC Number：
CCL-11™
- 年限：
82 days
- 组织来源：
subcutaneous connective tissue; areolar and adipose
- 库存：
大量
- 品系：
C3H/HeN
| Designations: | NCTC clone 2472 | ||
| Depositors: | KK Sanford | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Tissue: subcutaneous connective tissue; areolar and adipose Strain: C3H/HeN Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Virus Resistance: | poliovirus 3 | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | H-2k | ||
| Cytogenetic Analysis: | modal number = 52; range = 47 to 173, An extra long telocentric demonstrable in 100/100 cells. No metacentrics were observed. | ||
| Age: | 82 days | ||
| Gender: | male | ||
| Comments: | NCTC clone 2472 was derived in May, 1956, by K.K. Sanford, et al. from NCTC strain 1742 (Subline VII) of the parent NCTC clone 1328. [22230 ] The parent line was derived by G.D. Likely, et al. in November, 1950, from a culture of subcutaneous areolar and adipose connective tissue taken from a normal 82-day-old male C3H/HeN mouse. [26086 ] While cultures of NCTC strain 1742 produced sarcomas in 97% of the mice injected, cultures of NCTC clone 2472 under similar conditions produced sarcomas in 65% of the mice injected. [26087 ] NCTC clone 2472 cells have been cultured since origin in an antibiotic-free mixture of medium NCTC 109 (135), with 10% horse serum. NCTC clone 2472 has been compared with related clones, such as NCTC clone 2555 (ATCC CCL-12 ), and certain other cell lines with respect to neoplastic and physiologic properties, nutitional needs, karyology, enzyme activities, and rates of glycolysis. [22230 ] [26090 ] [26093 ] [26104 ] [26106 ] Plating efficiency is approximately 10% in the recommended culture medium. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: NCTC 135 medium, 90%; horse serum, 10% Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 3 times per week Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2040 | ||
| References: | 22230: Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477 26086: Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507 26087: Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880 26090: Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337 26093: Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776 26104: Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340 26106: . . Exp. Cell Res. 28: 381, 1962. |
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NCTC clone 2472
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