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- 文献和实验
- 技术资料
- ATCC Number:
CRL-1775™
- 生长状态:
悬浮生长
- 免疫类型:
IgG1
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 细胞形态:
淋巴样
- 运输方式:
冻存运输
- 库存:
大量
| Designations: | HP6025 | ||
| Depositors: | CB Reimer | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human IgG4 (Fc) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the Centers for Disease Control, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Hybridoma 3:263-275, 1984). | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with purified human myeloma immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 10 mM HEPES and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 58066: Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201 58067: Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923 |
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文献和实验惠普(HP)是一家立足于进步和创新的高科技企业。作为惠普(HP)的核心研究机构,惠普实验室担负着开创公司未来的重任。 惠普实验室的职责 惠普的大多数研究工作都有其业务集团主导,主要是开发各种产品和服务,以满足客户需求。 惠普实验室的职责是开发新的突破性技术和技术进步,为惠普(HP)增加竞争优势,并在惠普(HP)现有的策略之外创造新的商机。 同时,实验室还负责惠普公司(HP)的战略制定和调整,并投资于惠普关注的基础科学和技术领域。
(1)原理: 在待测血清中加入一定量的血红蛋白液,使之与待测血清中的结合珠蛋白(Hp)形成Hp-Hb复合物。通过电泳法将结合的Hp-Hb复合物与未结合的Hb分开,测定Hp-Hb复合物的量,从而得到血清中结合珠蛋白的含量。 参考值:0.8~2.7g/L. (2)临床意义: 增高见于妊娠、慢性感染、恶性肿瘤等,但不能排除溶血;减低见于各种溶血、肝病或无结合珠蛋白血症、巨幼细胞贫血等。
现行Hp检测可能出现的假阳性是检验技师考试中所包含的内容。医学教育网收集整理了部分相关信息供学员参考。 意大利Brandi等报告,在低酸患者胃液及胃黏膜中发现非幽门螺杆菌(Hp)尿素酶阳性细菌。该结果表明,在临床上对低酸患者采用胃黏膜快速尿素酶试验(RUT)及尿素呼气试验(C-UBT)诊断Hp感染,可能会有假阳性。Hp是迄今所知的唯一在人胃中携带活跃尿素酶的细菌。因此,胃黏膜RUT及C-UBT被用于快速检测Hp感染。但有报告发现,胃切除术后的患者中存在C-UBT假
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