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- 2025年09月19日
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- 询价记录
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- 器官来源：
肝
- 品系：
C57L
- 库存：
大量
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 相关疾病：
肝癌
- ATCC Number：
CRL-2026™
| Designations: | Hepa-1c1c7 | ||
| Depositors: | O Hankinson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Strain: C57L Disease: hepatoma |
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| Cellular Products: | cytochrome P450IA1 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Receptors: | aryl hydrocarbon (Ah) | ||
| Comments: | This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. The cells are highly inducible for cytochrome P450IA1. This and mutant derivatives of it are useful for studying the aryl hydrocarbon (Ah) receptor and P450IA1 regulation. |
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| Propagation: | ATCC complete growth medium: Alpha minimum essential medium without nucleosides, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 18 hrs | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 22320: Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. PubMed: 4362668 22342: Hankinson O, et al. Genetic and molecular analysis of the Ah receptor and of Cyp1a1 gene expression. Biochimie 73: 61-66, 1991. PubMed: 1851644 22593: Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390 |
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文献和实验） 通过建筑物的排风系统排到建筑物外面；（c） 直接排到建筑物外面。HEPA过滤器可以装在生物安全柜的压力排风系统（the exhaust plenum）里，也可以装在建筑物的排风系统里。有些Ⅰ级生物安全柜装有一体式排风扇，而其他的则是借助建筑物排风系统的排风扇。Ⅰ级生物安全柜是最早得到认可的，并且由于其设计简单，目前仍在世界各地广泛使用。Ⅰ级生物安全柜能够为人员和环境提供保护，也可用于操作放射性核素和挥发性有毒化学品。但因未灭菌的房间空气通过生物安全柜正面的开口处直接吹到工作台面上，因此Ⅰ级生物
实验步骤 Generating labeled coa standards (1) Thaw frozen cells directly into the SILEC expansion medium. (2) For Hepa 1c1c7 (adherent) cells, allow cells to reach confluence (3–4 d), wash two times with PBS
。 检查项目包括： a)风速、风量试验。 b)密闭度试验。 c)HEPA过滤器性能试验。 (2)实验室的设计 1)在实验专用之建筑物范围或在建筑物内，要明确划分出一实验区域，限制实验人员以外的人*近该地区。 2)实验区域之入口需有缓冲室，其前后二扇门不能同时开启。而且应有更衣室及沐浴室设备。 3)将试验材料及其它物品搬入实验区域时
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