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- 文献和实验
- 技术资料
- 生长状态：
悬浮生长，少量贴壁
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-13006™
- 器官来源：
脾
- 库存：
大量
- 运输方式：
冻存运输
- 免疫类型：
IgG1 kappa
- 细胞形态：
淋巴样
| Designations: | 6.12 | ||
| Depositors: | ImClone Systems Inc. | ||
| Isotype: | IgG1 kappa | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, with some loosely adherent cells | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Organ: spleen Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The antibody specifically binds to an extrcellular domain of human vascular endothelial growth factor (VEGF) receptor 1 [fms-like tyrosine kinase (Flt-1) [U.S. Pat. 6,365,157; U.S. PAT. 6,448,077]. The 6.12 cell line was deposited at the ATCC as PTA-3344. Progeny from PTA-3344 are available as CRL-13006. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml. Adherent cells may be removed by shaking the flask. Interval: Maintain cell density between 3 X 10(4) and 4 X 10(5) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 70143: Rockwell P, Goldstein NI. Monoclonal antibodies specific to VEGF receptors and uses thereof. US Patent 6,365,157 dated Apr 2 2002 70144: Rockwell P, Goldstein NI. Chimeric and humanized monoclonal antibodies specific to VEGF receptors. US Patent 6,448,077 dated Sep 10 2002 |
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文献和实验也可用辅酶型维生素B 12 、辅酶 B 12 等词来表示。是由 5- 脱氧腺苷基取代氰钴胺素（ CN － B 12 ）中与钴连接的 CN 基而生成的，所以亦称钴胺酰胺辅酶。其中大多数是核苷酸中的碱基为 5 ， 6- 二甲（基）苯并咪唑的二甲（基）苯并咪唑钴酰胺辅酶（ dimeth － ylbenzimidazole cobamide coenzyme ）和钴胺素辅酶，但也有碱基为腺嘌呤或 5- 羟基苯并咪唑所置换的 B 12 辅酶。 B 12 辅酶是 H.Barker 等在研
丁香园myl0605的观点：前几天为自己养的PC12是未分化还是分化型困扰,查了一些资料,现发上来希望能给有同样问题的战友有一点帮助!培养条件:ＰＣ12细胞培养于铺有鼠尾胶原的玻璃培养瓶中,置于ＣＯ2培养箱(37℃,95%空气5%ＣＯ2),培养基选用ＤＭＥＭ(ｐＨ7.4,高糖),加入10%灭活小牛血清及5%灭活马血清。分化前:ＰＣ12细胞在培养基中多呈圆形,直径15～20μｍ,也有椭圆或多角形的,扁平的细胞少见,倾向于聚集成小团簇状(A图)。分化后:加入ＮＧＦ后的24ｈ内细胞停止分裂,长出似
人 apelin 1 2 (AP1 2 ) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 apelin 12(AP12) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 apelin 1 2 (AP1 2 ) 水平。用纯化的 抗- apelin 1 2 (AP1 2 ) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中
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