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- 2026年02月20日
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- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 库存：
大量
- ATCC Number：
HB-8484™
| Designations: | 454C11 | ||
| Depositors: | Cetus Corp. | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human breast cancer cells | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized either with live cells or with membrane extracts from human breast cancer cell lines. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue. By immunofluorescence, the antibody reacts with epithelia of several normal and neoplastic tissues. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 3895: Ring DB, et al. Monoclonal anti-human breast cancer antibodies. US Patent 4,753,894 dated Jun 28 1988 | ||
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文献和实验454测序仪的出现极大促进了测序业务的开展，科研人员已经将测序技术作为解决科研工作中许多常见 问题的利器。这是因为454测序仪在以下几个方面取得了质的突破：首先是解决了高通量测序问题；其次它简 化了样品准备步骤，将以往转化大肠杆菌扩增质粒的繁琐过程全部用简单的体外PCR扩增法替代了；最后， 它缩小了测序反应体积，节省了 试剂 。这样，454测序仪做到了以极其低廉的价格进行大规模平行测序反应。 它的测序规模之大、测序费用之低
454 Life Sciences is a biotechnology company based in Branford, Connecticut specializing in high-throughput DNA sequencing using a novel massively parallel sequencing-by-synthesis approach. 454 has experienced rapid growth since its partnership
Roche 454（GS FLX Titanium System）超高通量测序技术原理
2005年底，454公司推出了革命性的基于焦磷酸测序法的超高通量基因组测序系统——Genome Sequencer 20 System，被《Nature》杂志以里程碑事件报道，开创了边合成边测序的先河。2007年又推出了性能更优的第二代基因组测序系统——Genome Sequencer FLX System。2008年10月，454推出了全新的GS FLX Titanium系列试剂和软件，让GS FLX的通量一下子提高了5倍，准确性和读长
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