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- 文献和实验
- 技术资料
- 器官来源：
胚胎
- 生长状态：
贴壁生长
- 库存：
大量
- 细胞形态：
成纤维样
- ATCC Number：
CRL-1503™
- 年限：
embryo
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
成纤维细胞
- 品系：
SIM
- 运输方式：
冻存运输
| Designations: | STO | ||
| Depositors: | G Martin | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo Strain: SIM Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | The STO cell line was derived by A. Bernstein, Ontario Cancer Institute, Toronto Ontario, Canada from a continuous line of SIM mouse embryonic fibroblasts. | ||
| Applications: | transfection host | ||
| Age: | embryo | ||
| Comments: | The STO cell line was derived by A. Bernstein, Ontario Cancer Institute, Toronto, Canada from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. The cell line is used routinely to prepare feeder layers by irradiation or mitomycin C treatment. Such populations are then employed for maintenance of stock teratocarcinoma stem cells (see ATCC CRL-1535 and CRL-1566) in the undifferentiated state.The cells have been tested and found negative for ectromelia virus (mousepox). | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 irradiated to be used as feeder cells:ATCC 56-X |
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| References: | 1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416 21874: . Teratomas and differentiation. New York: Academic Press; 1975. 22387: . . Cell 6: 467-474, 1975. 22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624 |
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文献和实验。 我对此专门做过测试，RT下放1小时，基本无影响，4小时后开始有降解。 4hr－RT表示在RT下放 4hr-RT-sto表示在RT下放在我研制的RNA保护剂中放置4小时 husttjmu zjubell，非常感谢你的帮助！我心里有底啦，谢谢！ 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你，都可以成为师兄的好伙伴 师兄微信号：shixiongcoming
ES Cell Culture and Manipulation
them when a colony is approximately 1-2 times the diameter of a stretched out fibroblast (STO cell). If the plate is densely covered with colonies, they may be split 1:10, or slightly greater. If there are a lot of colonies, split them 1:6-1:8. For a plate
ES Cell Culture and Manipulation
(STO cell). If the plate is densely covered with colonies, they may be split 1:10, or slightly greater. If there are a lot of colonies, split them 1:6-1:8. For a plate that has only a few colonies (12-20), allow them to get slightly larger than normal
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