- 询价
- 2026年06月27日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 器官来源：
外周血
- 年限：
54 years
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 库存：
大量
- ATCC Number：
CRL-5953™
- 细胞形态：
淋巴样
| Designations: | NCI-BL1607 [BL1607, BL9, NCI-BL9] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
||
| Source: | Organ: peripheral blood Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10 D13S317: 9,12 D16S539: 11,13 D5S818: 11,12 D7S820: 8,12 THO1: 9.3 TPOX: 8,11 vWA: 17 |
||
| Age: | 54 years | ||
| Gender: | male | ||
| Ethnicity: | White | ||
| Comments: | NCI-BL1607 is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from a lymph node metastasis from a patient with primary small cell lung cancer. The patient was a smoker. 20 pack years. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(6) viable cells/ml. Maintain cell density between 1 X 10(6) and 3 X 10(6) viable cells/ml. |
||
| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
||
| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1、 首先安装 BL-420F 系统应用软件，安装完成后，驱动程序被复制到硬盘上，注意，你在光盘上是找不到驱动程序的。 安装应用软件后设备驱动程序在硬盘上的位置 2、 如果是原来已经安装驱动程序，但是驱动程序安装有问题，此时你需要更新驱动程序，更新的方法是：在「我的电脑」上单击右键弹出快捷菜单，选择属性，将弹出系统属性对话框，参见下图。 系统属性对话框 3、 在「系统属性」对话框中选择顶部的「硬件」栏，然后单击「设备管理器」按钮弹出「设备管理器」对话
一个新的有趣的方法是基于磁性细菌Magnetospirillum magneticum建立的细菌磁性颗粒（BMPs）。通过融合蛋白技术，在细菌表面BMPs展示了其特异的蛋白 性能。这个生物技术工具，组合了磁性和生物特异结合特性，可以应用于全自动的CL免疫分析，也可用于核酸的纯化和分析。 生物发光共振能量转移（Bioluminescence Resonance Energy Transfer）最近，人们把注意力集中到了活细胞的共振
动物来源：1921年C.C.Little用Lathrop小鼠作近亲培育时，用No.57雄鼠和No.52雌鼠交配，培育成C57。在C57中将毛色呈黑色的进行固定，培育成C57BL。1937年Little将维持的C57BL第六组亚系定名为C57BL/6，将第十组亚系定名为C57BL/10，继而分别维持至今。1947年从Little引入到美国Jackson Lab.，形成C57BL/6J；1951年从Jackson Lab.引到NIH，形成C57BL/6N亚系。六十年代到日本实验动物中央研究
技术资料暂无技术资料 索取技术资料
