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- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 库存：
大量
- 相关疾病：
肝癌
- 器官来源：
肝
- ATCC Number：
CRL-1548™
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
上皮样
| Designations: | H-4-II-E | ||
| Depositors: | JA Bradlaw | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Disease: hepatoma |
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| Cellular Products: | aryl hydrocarbon hydroxylase is inducible | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The line has been cured of Mycoplasma arginini contamination. The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 4196: Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894 22186: . . In Vitro 16: 230, 1980. 23140: Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181 58038: Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836 58076: Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233 |
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文献和实验E L I S A w i t h p l a t e l e t s
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
E.Z.N.A. Plant RNA Midi Protocol II (for difficult samples)
ml collection tube (Not supplied), and add 3.5 ml RNA Wash Buffer II diluted with ethanol. Centrifuge at 4,000 x g for 5 minutes at room temperature and discard flow-through. Reuse the collection tube in step 11. 11. Wash column with a second 3 ml of RNA
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
yields. 3. Transfer the sample to a 1.5 ml centrifuge tube. Add 500 ul Solution II and mix gently but throughly by inverting and rotating the tube 7-10 times to obtain a cleared lysate. This may require a 2-3 min incubation at room temperature
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