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- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 细胞类型：
上皮细胞
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 相关疾病：
肝癌
- 器官来源：
肝
- 库存：
大量
- ATCC Number：
CRL-2712™
- 品系：
C57L/J
| Designations: | vT{2} | ||
| Depositors: | O Hankinson | ||
| Biosafety Level: | 2 [Cells contain SV40 and CMV viral DNA ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as mouse | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Strain: C57L/J Disease: hepatoma Cell Type: epithelial |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The vT{2} cell line was derived by co-transfection of a 6 thioguanine-resistant derivative of c4 (B13NBii1) [ATCC CRL-2717 ] cell line using the plasmid pSV2gpt and pBM5/NEO-M1-1. M1-1 is a cDNA clone containing the entire human ARNT cDNA sequence. The cells were expanded in G418 to obtain vT{2} (ATCC CRL-2712 ). The vT{2} cell line expresses the human aryl hydrocarbon receptor nuclear translocator (ARNT) gene The vectors contain cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. ARNT is directly involved in the regulation of xenobiotic metabolism (including chemical carcinogenesis), hypoxia and differentiation during embryogeneses. The parental cell line c4 (B13NBii1) (ATCC CRL-2717 ) lacks functional ARNT while its derivative vT{2} (ATCC CRL-2712 ) possesses a complete transfected ARNT cDNA. Together, they can be used to study ARNT processes and the role of ARNT in vivo. | ||
| Propagation: | ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine supplemented with 0.4 mg/ml G418, 90%; heat-inactivated fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended Medium Renewal: Every 2 to 3 day |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | parental cell line:ATCC CRL-2717 | ||
| References: | 61153: Hoffman EC, et al. Cloning of a factor required for activity of the Ah (dioxin) receptor. Science 252: 954-958, 1991. PubMed: 1852076 | ||
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文献和实验Escherichia coli O157:H7 is a highly virulent pathogen that causes severe food poisoning in humans. Many outbreaks involving this pathogen have been linked to minced beef. A protocol is presented here to detect E. coli O157:H7 in minced beef
811光电比色计的放大电路偶尔也会出现这样或那样的故障，导致工作失常。现将该机放大电路的几例常见故障及其检修方法介绍如下，供参考。故障现象一：透射比T调零失灵。调节透射比T旋钮不起作用，甚至调不到T=0。分析检修：根据故障现象分析，引起该故障的主要原因是： (1)截光器损坏，导致无法调节透射比； (2)调零电位器损坏，使得调零失灵； (3)前置放大器或透射比放大器工作失常。检查截光器完好，测量调零电位器阻值变化正常，由此说明，故障在前置放大器或透射比放大器电路中。前置放大器由场效应管VT
因子当然在核内，这是一个很大的蛋白类群,人类中已知的转录因子就有上千个。 提供一本电子书下载，里面对已知人类基因进行了分类，给出了几乎所有已知的转录因子。 http://www.brsbox.com/filebox/uploadcomplete/randstr/E4u0VT256148924eQb05Wy472m/fgid/d931ad476c444f8cf5c5279fe8694b4d/dirids/ luoqq1985 转录因子有很多，关于其调控的研究
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