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- 2026年06月27日
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- 详细信息
- 技术资料
- 相关疾病：
其他疾病
- ATCC Number：
CCL-129™
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 器官来源：
脾
- 库存：
大量
- 运输方式：
冻存运输
- 细胞形态：
上皮样
| Designations: | VSW | ||
| Depositors: | HF Clark | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Vipera russelli | ||
| Morphology: | epithelial |
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| Source: | Organ: spleen Disease: tumor, metastatic |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit | ||
| Isolation: | Isolation date: 1967 | ||
| Virus Susceptibility: | Vesicular stomatitis virus Rabies virus Human poliovirus 3 |
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| Cytogenetic Analysis: | model number = 64; range = 33 to 118. Karyotype slightly unstable with stemline number in hypertriploid range. Cells with modal numbers of 28 and 29 macrochromosomes and 34 to 37 microchromosomes. Many cells with a very small chromosome with a median centromere included with macrochromosomes. All cells have 2 to 3 marker chromosomes with terminal centromeres. | ||
| Gender: | female | ||
| Comments: | The cells produce a C type retrovirus. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 30.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Once per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 22232: Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059 26297: Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585 26298: Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139 |
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VSW
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