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- 文献和实验
- 技术资料
- 库存：
大量
- ATCC Number：
HB-327™
- 生长状态：
混合型生长
- 免疫类型：
IgG2b
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
| Designations: | 4C4 | ||
| Depositors: | A Bendelac | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed: adherent and suspension, The cells are rather adherent in nature, and grow best if maintained in suspension culture plates (e.g., Corning Catalog number 430591). | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against mouse CD1.1 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected). Spleen cells were fused with Sp2/0-Ag14 cells. The antibody blocks recognition of CD1.1 by some, but not all T cells. The antibody reacts with CD1.1, and cross-reacts with CD1.2. The epitope recognized by this antibody is distinct from that recognized by the cluster represented by 15F7 (ATCC HB-322 ) and 20H2 (ATCC HB-323 ). The antibody performs well in flow cytometry applications. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 95%; fetal bovine serum, 5% Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Maintain cultures at cell concentrations between 4 X 10(5) and 8 X 10(5) viable cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 38507: Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266 38508: Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267 38509: Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714 |
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文献和实验相关实验
的3%，使用这一途径。c4C4途径的进化涉及叶片解剖、基因表达、细胞生物学和生物化学的改变。但是C4植物比C3植物有竞争优势：它们使用的水少了3倍，可以在干旱、高温和二氧化碳限制的条件下生长。许多研究人员现在正致力于了解C3和C4植物之间的差异，试图设计出更节能和节水的重要作物。只有四种主要作物使用C4光合作用：玉米、甘蔗、高粱和小米。然而，一些生物工程项目正在进行中，目的是提高粮食和生物燃料的生产率。为了了解C4代谢的进化和如何进行基因工程，我们需要从密切相关的C3和C4物种获得基因组和转录组信息。C3
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4C4
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