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- 技术资料
- 生长状态：
悬浮生长
- 免疫类型：
IgG2a
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- ATCC Number：
CRL-2284™
| Designations: | 3C5.1 | ||
| Depositors: | S Linn | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human DNA polymerase epsilon (pol epsilon) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with Mono S FPLC purified DNA polymerase epsilon. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon. It does not recognize DNA polymerases alpha, beta or delta. The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon. |
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| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM hypoxanthine and 0.008 mM thymidine, 80%; fetal bovine serum, 20% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
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| References: | 24404: Chui G, Linn S. Further characterization of HeLa DNA polymerase epsilon. J. Biol. Chem. 270: 7799-7808, 1995. PubMed: 7536191 | ||
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文献和实验如下化学反应： H2O＋SO2＋I2＋3C5H5N→2C5H5N·HI＋C5H5N·SO3 C5H5N·SO3+CH3OH→C5H5N·HSO4CH3 C5H5N·HI→C5H5N·H++ I- 该反应持续进行，不断消耗水，生成I-，一直到反应滴定终点，水分消耗完毕。这时，溶液有微量未发生反应的卡尔费休试剂存在，才能发生I2和I-同时存在的情况，两个铂电极之间的溶液开始导电，由电流指示达到终点，停止滴定。从而通过计量已消耗的卡尔费休试剂体积（容量）来标定溶液中的水分含量。 卡尔
一、原理：本仪器为卡尔-费休（Kart Fischer）容量滴定法测定水份含量的仪器，采用“永停法”来确定终点。根据半电池反应：I2＋2e2Iˉ，溶液中同时存在I2及Iˉ时上述反应分别在两个电极上进行，即在一个电极上I2被还原，而再另一个电极上Iˉ被氧化，因此在两个电极之间有电流通过。如果溶液中只有Iˉ而无I2则电极间无电流通过。当滴定终点时溶液中有微量卡尔-费休试剂存在，即有Iˉ及I2同时存在，这时溶液导电，仪器显示滴定到达终点。反应式：I2+SO2+3C5H5N+CH3OH+H2O→2C
。对 Vibrio cholera 神经氨酸苷酶和 Pasturella hemolytica 起源的糖蛋白酶敏感的称之为 I 类位点，针对此位点的抗体克隆号有： MY10 ， B1.3C5 ， 12.8 和 ICH3 。对唾液酸不敏感但能与 PhG 粘附的称之为 II 类位点，识别此位点的抗体是 QBEND10 。对以上几种酶都耐受的，称之为 III 类位点，可被8G12 、 TUK3 、 115.2 等克隆号的抗体可识别。 使用 III 类位点特异性抗体能最大限度地提高检测的敏感度。这是因为抗体与荧
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