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- 生长状态：
悬浮生长
- 免疫类型：
IgM; kappa light chain
- 细胞形态：
淋巴样
- 库存：
大量
- ATCC Number：
CRL-2440™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
| Designations: | 3A5 | ||
| Depositors: | PJ Bjorkman | ||
| Isotype: | IgM; kappa light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against HFE | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The cell line was established by Jose A. Lebron and Pamela J. Bjorkman in 1997. Animals were tolerized with human beta2-microglobulin and immunized with purified, soluble, human HFE. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). The antibody recognizes the HFE portion of the HFE/beta2 heterodimer. HFE is a major histocompatibility complex (MHC) related protein that is mutated in the iron-overload disease hereditary hemochromatosis. This antibody can be used for ELISA applications and to immunoprecipitate soluble HFE; it is not reactive in Western Blot assays. |
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| Propagation: | ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201). Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp4 viable cells/ml. Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed. |
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| Preservation: | HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%. | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 39791: Lebron JA, et al. Tolerization of adult mice to immunodominant proteins before monoclonal antibody production. J. Immunol. Methods 222: 59-63, 1999. PubMed: 10022372 39792: Lebron JA, et al. Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Cell 93: 111-123, 1998. PubMed: 9546397 |
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文献和实验Thin-Layer Chromatographic Analysis of Human CYP3A-Catalyzed Testosterone 6-Hydroxylation
At least two cytochromes P450 belonging to the CYP3A subfamily may be expressed in adult human liver (1 ), CYP3A4 and CYP3A5. A third enzyme, CYP3A7, is expressed in human fetal liver (2 ). The CYP3A enzymes account for an estimated ~30
【原创】请教高手，我的这种设计不同种属的同源基因引物的方法是否正确？
好的引物，而且有说明该引物适合于哪些物种，这里说道还适合大鼠的，但是没有说适合小鼠的。反正就是根据这种方法，改变前面选的不同的基因和后面不同的pcr，应该可以找到适合不同种属的引物。 这里还可以清楚的看到，三种种属该基因的同源序列： http://www.ncbi.nlm.nih.gov/mapview/maps.cgi?taxid=10116&chr=5&MAPS=genes-r-org/human-chr/rat%3A5,genes-r-org/mouse-chr/rat%3A5,genes-r-org
Genotyping for Cytochrome P450 Polymorphisms
, and (*6 alleles), and CYP3A5 ((*3 allele).
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