- 详细信息
- 技术资料
- 细胞形态:
成纤维样
- 运输方式:
冻存运输
- ATCC Number:
CRL-10907™
- 库存:
大量
- 器官来源:
肝
- 生长状态:
贴壁生长
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 组织来源:
stroma
- 年限:
fetus
- 细胞类型:
其他细胞类型
| Designations: | 2018 | ||
| Depositors: | ImClone Systems Inc. | ||
| Biosafety Level: | 2 [CELL CONTAIN SV40 VIRAL DNA SEQUENCES ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: liver Tissue: stroma Cell Type: fibroblast immortalized with SV40 large T antigen |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims. ATCC is required to inform the Patent Depositor of the party to which the material was furnished. |
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| Age: | fetus | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 32.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every two to three days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| References: | 70741: Lemischka IR. Nucleic acids encoding hencatoporetic stem cell receptor flk-2. US Patent 5,185,438 dated Feb 9 1993 70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993 70743: Lemischka IR. Nucleic acids encoding hematopoietic stem cells receptors flk-1. US Patent 5,283,354 dated Feb 1 1994 70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994 70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996 70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997 70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998 70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999 |
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