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- 2025年10月17日
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- 详细信息
- 技术资料
- 生长状态：
悬浮生长
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- ATCC Number：
CRL-9300™
- 细胞形态：
淋巴样
- 库存：
大量
- 器官来源：
脾
- 运输方式：
冻存运输
| Designations: | 20H11 | ||
| Depositors: | Genetic Systems Corp. | ||
| Biosafety Level: | 2 [Cells Contain HERPESVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: spleen Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed |
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| Cellular Products: | immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa (flagella type b) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Peripheral blood B cells from a patient immunized with high molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus. The antibody reacts with P aeruginosa Fisher immunotypes 2, 6 and 7. The antibody cross-reacts extensively with clinical isolates of P. aeruginosa bearng type b flagella. |
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| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15% |
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| Subculturing: | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Doubling Time: | 24 to 36 hrs | ||
| References: | 3871: Rosok MJ, Lostrom ME. Monoclonal antibodies to Pseudomonas aeruginosa flagella. US Patent 4,834,976 dated May 30 1989 | ||
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20H11
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