immunoglobulin; monoclonal antibody; against a synthetic ras oncogene peptide
Permits/Forms:
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Comments:
Animals were immunized with a synthetic polypeptide with the sequence YTLVREIRQHKLRKLNPPDESGPGC corresponding to peptides 157 to 181 of the H-ras oncogene. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with the synthetic H-ras oncogene peptide. It reacts with the Harvey (Ha-ras) p21 protein but not the Kirsten (Ki-ras) p21 protein.
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation:
culture medium 95%; DMSO, 5%
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References:
21967: Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991 55710: Pumiglia K, et al. Raf-1 N-terminal sequences necessary for Ras-Raf interaction and signal transduction. Mol. Cell. Biol. 15: 398-406, 1995. PubMed: 7799948