- 询价
- 2026年03月22日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 相关疾病：
正常
- 器官来源：
肾脏
- ATCC Number：
CCL-139™
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 细胞类型：
SV40转化细胞
- 年限：
weanling
- 生长状态：
贴壁生长
- 品系：
C3H/Mai
- 细胞形态：
上皮样
| Designations: | TCMK-1 | ||
| Depositors: | PH Black, W Rowe | ||
| Biosafety Level: | 2 [Cells contain polyomavirus DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial |
||
| Source: | Strain: C3H/Mai Organ: kidney Disease: normal Cell Type: SV40 transformed |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1963 | ||
| Virus Susceptibility: | Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1 |
||
| Virus Resistance: | poliovirus 1 | ||
| Tumorigenic: | No | ||
| Antigen Expression: | H-2k | ||
| Cytogenetic Analysis: | modal number = 78; range = 64 to 157. All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes. |
||
| Age: | weanling | ||
| Comments: | The cells are SV40 transformed and are positive for SV40 T-antigen. About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable. Tested and found negative for ectromelia virus (mousepox). |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
||
| References: | 22273: Black PH, Rowe WP. SV-40 induced proliferation of tissue culture cells of rabbit, mouse, and porcine origin. Proc. Soc. Exp. Biol. Med. 114: 721-727, 1963. PubMed: 14120333 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
TCMK-1
询价
