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- 文献和实验
- 技术资料
- 免疫类型：
IgG2b
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 生长状态：
悬浮生长
- ATCC Number：
TIB-165™
| Designations: | 10-1.D.2 | ||
| Depositors: | DE Mosier | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against Lyb 2.1 mouse B cell (B lymphocyte) antigen | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with spleen cells from DBA/2 mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Lyb-2.1 antigen is a mouse B cell differentiation antigen (45000 dalton glycoprotein). The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells. B cells activated in this way mature into immunoglobulin secreting cells in the presence of T cell replacing factors (medium from Con A treated T cells). Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 968: Subbarao B, Mosier DE. Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody. J. Immunol. 130: 2033-2037, 1983. PubMed: 6601145 | ||
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