immunoglobulin; monoclonal antibody; against platelet derived growth factor B chain (PDGF B, v-sis form)
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Tumorigenic:
Yes
Comments:
Animals were immunized with affinity purified PDGF B v-sis. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the B chain of PDGF v-sis.
ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation:
fetal bovine serum, 90%; DMSO, 10%
References:
41495: Thomason AR, Nicolson MA. Methods for purification of platelet-derived growth factor. US Patent 5,175,255 dated Dec 29 1992