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- 细胞形态：
淋巴样
- 库存：
大量
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 器官来源：
脾
- 相关疾病：
其他疾病
- ATCC Number：
CRL-8155™
- 年限：
5 years
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
| Designations: | WIL2-NS | ||
| Depositors: | TA Coons | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: spleen Disease: hereditary spherocytosis Cell Type: B lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 9,12 F13A01: 6,7 F13B: 10 FESFPS: 11,12 LPL: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 17,20 |
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| Age: | 5 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line does not secrete immunoglobulin. The line was derived from the WIL-2 B cell line. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 22789: Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231 23199: Alspaugh MA, Tan EM. Antibodies to cellular antigens in Sjogren's syndrome. J. Clin. Invest. 55: 1067-1073, 1975. PubMed: 804494 26176: Vaughan JH, et al. Synthetic polypeptides and antibodies related to epstein-barr virus nuclear antigen. US Patent 4,654,419 dated Mar 31 1987 26177: Vaughan JH, et al. Assay for Epstein-Barr virus infection with solid phase bound synthetic polypeptides. US Patent 5,116,725 dated May 26 1992 26178: Vaughan JH, et al. Assay of anti-Epstein-Barr virus nuclear antigen antibodies with synthetic polypeptides. US Patent 5,122,448 dated Jun 16 1992 26179: Levy JA, et al. Further characterization of the WI-L1 and WI-L2 lymphoblastoid lines. J. Natl. Cancer Inst. 46: 647-654, 1971. PubMed: 4323138 |
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