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- ATCC Number：
CRL-2580™
- 免疫类型：
IgG
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
| Designations: | MR1 [derivative of HB-11048] | ||
| Depositors: | Bristol-Myers Squibb Pharm. Res. Inst. | ||
| Isotype: | IgG | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Organism: | Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against mouse CD40 ligand (CD154, CD40L, gp39) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with mouse T cells (Th1). Spleen cells were fused with NS-1 myeloma cells. The antibody detects a 39000 dalton protein selectively expressed on activated mouse T cells. A culture deposited at the ATCC as HB-11048 in May of 1992 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. | ||
| Preservation: | Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC HB-11048 |
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| References: | 23334: Noelle RJ, et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc. Natl. Acad. Sci. USA 89: 6550-6554, 1992. PubMed: 1378631 23483: Roy M, et al. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J. Immunol. 151: 2497-2510, 1993. PubMed: 8103067 |
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文献和实验相关实验
.5.3 Characteristic large zone of oxyhemoglobin Ao , plateau [Hb dimer] = 5.62 µM in 100 mM Tris⋅Cl, pH 7.40 ± 0.01/100 mM NaCl/1 mM Na2 EDTA at 21.5°C, run on Sephacryl S‐200. The centroid position, or V c , is defined as the position on the elution volume scale
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MR1 [derivative of HB-11048]
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