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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 品系：
BALB/c
- 相关疾病：
多发性骨髓瘤
- ATCC Number：
CCL-167™
- 生长状态：
悬浮生长
- 免疫类型：
IgG2b
- 细胞形态：
淋巴样
| Designations: | MPC-11 | ||
| Depositors: | MD Scharff | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Strain: BALB/c Disease: plasmacytoma; myeloma Cell Type: B lymphocyte; |
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| Cellular Products: | immunoglobulin; interleukin-6 (interleukin 6, IL-6) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Virus Resistance: | poliovirus 1 | ||
| Antigen Expression: | H-2d | ||
| Comments: | The cells contain A type viral particles. MPC-11 cells have been reported to produce murine IL-6. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 17 hrs | ||
| References: | 1039: Laskov R, et al. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. II. Assembly of IgG2b immunoglobulin by MPC 11 tumor and culture cells. J. Mol. Biol. 56: 327-339, 1971. PubMed: 5102530 1040: Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836 1041: Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146 22823: Blankenstein T, et al. DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma. J. Exp. Med. 171: 965-970, 1990. PubMed: 2106569 26031: Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284 |
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MPC-11
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