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- 详细信息
- 技术资料
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2060™
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 年限：
22 months
- 组织来源：
cerebral hemisphere
- 细胞形态：
成纤维样
- 器官来源：
大脑
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
| Designations: | PFSK-1 | ||
| Depositors: | D Fults | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: brain Tissue: cerebral hemisphere Disease: malignant primitive neuroectodermal tumor |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11 D13S317: 12,14 D16S539: 13 D5S818: 11,12 D7S820: 10 THO1: 9,9.3 TPOX: 8,9 vWA: 17,18 |
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| Cytogenetic Analysis: | hypotetraploid; 84, XXY; -Y, t(Xp;8q), del(1)(p22), -3, del(4)(p14), -9, -10, -13, -14, -14, -16, -22 | ||
| Age: | 22 months | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | PFSK-1 cells form colonies in soft agar, and lack contact inhibition. They express the intermediate filament protein, nestin, and are positive for neuron specific enolase (NSE). They lack characterisics of terminally differentiated neurons or glia. Restriction fragment length polymorphism studies showed loss of heterozygosity for multiple loci on chromosome 17. Neither c-myc nor N-myc is amplified or re-arranged. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin solution, add fresh trypsin - EDTA (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Doubling Time: | 30 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 23158: Fults D, et al. Establishment and characterization of a human primitive neuroectodermal tumor cell line from the cerebral hemisphere. J. Neuropathol. Exp. Neurol. 51: 272-280, 1992. PubMed: 1316433 | ||
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PFSK-1
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