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- 2026年07月29日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
NIH/Swiss
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 器官来源：
胚胎
- 细胞形态：
成纤维样
- 库存：
大量
- ATCC Number：
CRL-2187™
- 年限：
embryo
- 生长状态：
贴壁生长
| Designations: | PA317 cyclin E-L | ||
| Depositors: | JM Roberts | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo Strain: NIH/Swiss |
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| Cellular Products: | cyclin E-L | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | embryo | ||
| Comments: | PA317 cyclin E-L is an amphotropic retrovirus producing cell line constructed from the PA317 (see ATCC CRL-9078 ) fibroblast cell line by introduction of a retrovirus expression vector (LXSN). The LXSN vector uses the 5'long terminal repeat of Moloney murine sarcoma virus to express the inserted cDNA and contains the neomycin phosphotransferase gene (G418 resistance) as a selectable marker. The full length cDNA coding human cyclin E-L (1.7 kb HindIII fragment) was treated with Klenow fragment and cloned into the HpaI site of the vector. The PA317 cyclin E-L cell line expresses human cyclin E-L. Cyclin E is a nuclear protein essential for the G1 to S phase transition. Mammalian cells express two forms of cyclin E protein which differ from each other by the presence (cyclin E-L) or absence (cyclin E-S) of a 15 amino acid amino terminal domain. Fibroblasts engineered to constitutively overexpress either form of cyclin E show elevated cyclin E dependent kinase activity and a shortened G1 phase of the cell cycle. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose containing 0.1 mg/ml G-418, 90%; calf serum, 10% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Twice per week Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 23246: Ohtsubo M, et al. Human cyclin E, a nuclear protein essential for the G1-to-S phase transition. Mol. Cell. Biol. 15: 2612-2624, 1995. PubMed: 7739542 23508: Ohtsubo M, Roberts JM. Cyclin-dependent regulation of G1 in mammalian fibroblasts. Science 259: 1908-1912, 1993. PubMed: 8384376 |
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文献和实验干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
比高、实验周期短（ 1 天实现从细胞到文库构建）、可重复性好等显著优势，尤其适用于早期胚胎发育、干细胞、肿瘤以及表观遗传学等研究领域。 图 1. CUT&Tag 原理图 由于 CUT&Tag 主要是针对极低细胞起始量进行实验，因此对核心酶原料有着极高的要求。CUT&Tag 技术的核心原料酶是 Hyperactive PA/PG-Tn5 Transposase ，它具有高活性，对微量 DNA 有高灵敏度和高亲和力，能有效抓取数十个细胞中的有限结合位点等特点
人 前白蛋白(PA) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，组织，细胞上清及相关液体样本中前白蛋白(PA) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人前白蛋白 (PA) 水平。用纯化的人前白蛋白 (PA) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入前白蛋白 (PA) ， 再与 HRP 标记的前白蛋白 (PA) 抗体
大阪大学蛋白质研究所藤井勇树、高木淳一东北大学大学院医学系研究科金子美华、加藤幸成 前言在分析蛋白质的功能时最重要的是如何得到高纯度的蛋白。现今提纯蛋白最常用的方法是亲和标签系统。被统称为「Epitope Tag」的肽标签以及其单克隆抗体组成的系统有FLAG、HA、Myc等多种标签。但是，每个标签系统都有优缺点，并不存在完美的标签系统。于是，在克服了众多难关后我们终于开发出有超高亲和性的新型亲和标签系统“PA Tag System”1）。 PA tag的起源Epitope Tag
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