- 询价
- 2026年05月29日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
褐鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 库存：
大量
- ATCC Number：
CRL-8164™
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
| Designations: | BF-11 | ||
| Depositors: | University Patents, Inc. | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human erythropoietin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with human urinary erythropoietin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds alpha, beta and asialo forms of human erythropoietin. The antibody has been given the name Epoclonalan. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 1666: Weiss TL, et al. Characterization of a monoclonal antibody to human erythropoietin. Proc. Natl. Acad. Sci. USA 79: 5465-5469, 1982. PubMed: 6957875 3924: Goldwasser E, et al. Monoclonal anti-erythropoietin. US Patent 4,558,005 dated Dec 10 1985 |
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文献和实验11-脱氧皮质〔甾〕酮 11-deoxycorticosterone
C21 H30 O3 ， 21-羟基孕烯 -4-二酮 -3， 20。也单称脱氧皮质〔甾〕酮，或缩写 DOC。是副肾皮质激素的一种，具有糖代谢作用。由副肾皮质细胞的微粒体的 21-羟化酶的作用，从黄体酮进行生物合成的。其乙酸酯缩写为 DOCA，应用于临床。由于在副肾皮质细胞的线粒体中的 11β -羟化酶的作用可生成皮质酮，所以通常也认为是一种中间体。
1、原理与方法 基本上和C4高压电泳及免疫固定技术一致，样本不需经神经氨酸酶和羧肽酶B处理，电泳时直接加10uL EDTA抗凝血浆。凝胶板规格为125mm×260mm×3mm，电泳时电压控制在85mA，时间3～4h或血红蛋白迁移至离加样孔5.5em处时，停止电泳。用1：4或1：2稀释的抗Df血清(AtlanticAntibodiesTATB，USA或上海长征医院产品)进行免疫固定。依照Mauff提出的BF定型图命名。 2、所需试剂 （1）巴比妥缓冲液 巴比妥34.56g，NaOH6.00g
取代dGTP对扩增的成功是有帮助的。 11、引物应具有特异性。 引物设计完成以后，应对其进行BLAST检测。如果与其它基因不具有互补性，就可以进行下一步的实验了。 值得一提的是，各种模板的引物设计难度不一。有的模板本身条件比较困难，例如GC含量偏高或偏低，导致找不到各种指标都十分合适的引物；用作克隆目的的PCR，因为产物序列相对固定，引物设计的选择自由度较低。在这种情况只能退而求其次，尽量去满足
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