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- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 细胞类型：
成纤维细胞
- 生长状态：
贴壁生长
- 库存：
大量
- 器官来源：
皮肤
- 细胞形态：
成纤维样
- 相关疾病：
正常
- ATCC Number：
CRL-1513™
- 年限：
4 years
| Designations: | CCD-42Sk | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
||
| Source: | Organ: skin Disease: normal Cell Type: fibroblast |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,13 D16S539: 9,11 D5S818: 12,13 D7S820: 11 THO1: 7,8 TPOX: 8,11 vWA: 15 |
||
| Age: | 4 years | ||
| Gender: | male | ||
| Comments: | The line was derived from normal skin of a child that died of multiple congenital defects. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
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文献和实验sapiens (human) LS 180 CL-188 Homo sapiens (human) LS 174T CRL-10624 Homo sapiens (human) CO 88BV59-1 CRL-11538 Homo sapiens (human) Co88BV59H21-2 CRL-11539 Homo sapiens (human) Co88BV59H21-2V67-66 CRL-1459 Homo sapiens (human) CCD-18
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