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- 技术资料
- 库存：
大量
- 相关疾病：
正常
- ATCC Number：
CCL-201™
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 器官来源：
肺
- 细胞类型：
成纤维细胞
- 细胞形态：
成纤维样
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 年限：
48 years
| Designations: | CCD-8Lu | ||
| Depositors: | GP Maxwell | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: lung Disease: normal Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | blood type A; Rh + | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 10,11 D5S818: 13 D7S820: 10,11 THO1: 7 TPOX: 8,9 vWA: 15,18 |
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| Cytogenetic Analysis: | normal human male; diploid; stable | ||
| Age: | 48 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Derived from normal tissue from patient who died of cerebral thrombosis. The cells senesce at about population doubling level (PDL) 40 to 42. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
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CCD-8Lu
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