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- 详细信息
- 技术资料
- 年限：
4 years
- 运输方式：
冻存运输
- 器官来源：
外周血
- 生长状态：
悬浮生长
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- 细胞类型：
T淋巴细胞
- 细胞形态：
淋巴样
- 相关疾病：
白血病
- ATCC Number：
TIB-195™
| Designations: | CEM-CM3 | ||
| Depositors: | MK Cathcart | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Disease: acute lymphoblastic leukemia Cell Type: T lymphoblast; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 10,12 D5S818: 12,13 D7S820: 10,13 THO1: 6,7 TPOX: 8 vWA: 18,19 |
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| Age: | 4 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line was derived from CCRF-CEM (see ATCC CCL-119 ) by selecting for resistance to 8-azaguanine. The line has been used as a fusion partner in the production of human-human T cell hybridomas. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.015 mg/ml 8-azaguanine, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-119 |
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| References: | 21845: Cathcart MK, Murakami MNonspecific suppression of antibody synthesis by products of human T lymphocyte hybridomas. Use of a newly selected parent fusion lineIn: Cathcart MK, Murakami MT cell hybridomasBoca Raton, FLCRC Presspp. 227-236, 1985 21857: . Human T lymphocyte hybridomas: production of nonspecific suppressor factors of antibody synthesis. Kyoto, Japan: 5th International Congress of Immunology; 1983. 22405: . . Clin. Res. 31: 734A, 1983. |
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CEM-CM3
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