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- 详细信息
- 技术资料
- 免疫类型：
IgM
- 细胞形态：
淋巴样
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- ATCC Number：
HB-8329™
- 生长状态：
悬浮生长
| Designations: | CH26-1352 | ||
| Depositors: | Cetus Corp. | ||
| Isotype: | IgM | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against double stranded DNA | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with synthetic polynucleotide homopolymers [poly(dA-dT)-poly(dA-dT) and poly(dI-dC)-poly(dI-dC)] then boosted with foreign native DNA (EcoR1 digested pBR322 DNA). Spleen cells were fused with FO myeloma cells. |
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| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 90%; DMSO, 10% | ||
| References: | 3557: Huang CM, Cohen SN. Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same. US Patent 4,623,627 dated Nov 18 1986 | ||
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CH26-1352
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