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ANTIBODY DILUENT - 250ML
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文献和实验Monoclonal Antibody Production
) (3) 250 ml bottles of 100X penicillin-streptomycin (4) 10 ml of 100X HAT selection solution (5) 10 ml of 100X HT solution (6) 10 sterile flat-bottomed 96-well plates 3. Assaying for Positive Clones Run an ELISA.html">indirect ELISA using
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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