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PI3-K 3-step HTRF assay kit, 1920 wells
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文献和实验to shear DNA to lengths between 200 and 1000 basepairs being sure to keep samples ice cold. 5,� Centrifuge samples (part A, step 7) for 10 minutes at 13,000 rpm at 4�C, and detected the OD260 of the lysates. 6, Sonicated lysates
RAT/MOUSE GROWTH HORMONE ELISA KIT
. Decant Wash Buffer and remove the residual amount from all wells by inverting the plate and tapping it smartly onto absorbent towels several times. Do not let wells dry before proceeding to the next step. If an automated machine is used for the assay
The ribonuclease protection assay (RPA)
) and prerun at 40 watts constant power for ~ 45 minutes, with 0.5X TBE as the running buffer. Gel temperature should be 50°C. 24. Flush the wells again with 0.5X TBE and load the samples (from Step 20). Also load a dilution of the probe set
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