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- 抗体英文名：
PureEpi Chromatin Prep&Optimization Kit
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文献和实验Chromatin Immunoprecipitation (ChIP)
for fragmenting chromatin for N-ChIP, whereas chromatin for X-ChIP is usually sonicated, sometimes in conjunction with MNase digestion. 5. One of the major limitations of ChIP is the need for antibodies that recognize the target protein
Native Chromatin Preparation and Illumina/Solexa Library Construction
to chromatin as histones, the chromatin should be cross-linked using formaldehyde and sonicated to 200- to 300-bp length. 22. Resuspend 20-22 x 106 purified human CD4+ T cells from Step 21 in 1 mL MNase digestion buffer kept at room
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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