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Anti-CD45 (hu), PerCP-Cy5.5, clone HI30
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
in PBS with 100 U/ml RNase. Analyze on any 488 nm argon- 630 nm HeNe or diode dual laser flow cytometer for PI and Cy5 fluorescence. Both PI and Cy5 should be analyzed in linear mode. Recipes Anti-cyclin D1 (Pharmingen cat. no. 14561A, clone G12
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20℃) cyclin staining
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH2 0 (stored at -20°C) cyclin staining buffer (1% BSA
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