27685-94-5;S45405-

Single tube confirmation PCR protocol

. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq

多年PCR经验总结

SYSTEM ================================ human gDNA 0.1 ug-1 ug E.Coli 10 ng-100 ng LamadaDNA 0.5 ng-5 ng Plasmid DNA 0.1 ng-10 ng =============================== 4. termperature （1）denaturation 常规是94度5分钟，GC Rich的摸板是95度5分钟，除了GC Rich外，常规

Single tube confirmation PCR protocol

concentration). - Label 20 thin-wall PCR tubes (5 for each isolate) and add the following primer pairs: A-B, A-kanB, C-D, kanC-D, and A-D. Tubes 1-5 are for isolate #1, 6-10 are for isolate #2, ..., and tubes 16-20 are for the wild-type control. Each of the 20