Bst2UI内切酶北京现货

特别提示：包括Bst2UI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Bst2UI内切酶
英文名称：Bst2UI
产品货号：GS2087
产品规格：1kU

储存条件：冷冻（-20℃）

使用说明

To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl.
Recognition sites	CC↑WGG
来源	Bacillus stearothermophilus 2U
Assayed on	Lambda DNA
Optimal buffer	G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)+ BSA
Optimal temperature	60℃
Storage conditions	10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After Bst2UI overdigestion none of DNA fragmetns can be ligated.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 60℃.
Methlation effect	Not blocked by overlapping Dcm methylation(CmCWGG):CCWGG.
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	AjnI?^,BciT130I,BptI,BseBI,BsiLI,BstNI,BstOI,EcoRII^,MvaI,Psp6I?^,PspGI^

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