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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-70℃(Ships on Dry Ice)
- 保质期:
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- 英文名:
Tubulin, Porcine (rPeptide, Cat#T-1201-1)
- 供应商:
rPeptide
- 规格:
1.0 mg
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文献和实验Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have done. The protocol calls for 3 pig brains, and should yield ~ 60 mg of purified tubulin
Porcine brain tubulin preparation
protein is prepared. 16. A well-packed phosphocellulose column should be perfectly even in color with no evidence of "cracks" in the resin. The better the phosphocellulose column is packed, the more concentrated the peak of elution of tubulin protein
Purification of Tubulin from Porcine Brain
-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.
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