NSP-SA-NHS吖啶盐,199293-83-9

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NSP-SA-NHS

CAS No. : 199293-83-9

MCE 国际站：NSP-SA-NHS

产品活性：NSP-SA-NHS 是一种吖啶酯，可用于化学发光免疫分析。已经开发了一种以 NSP-SA-NHS 标记的 Bisphenol A (BPA) 的快速灵敏化学发光免疫测定方法。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: NSP-SA-NHS marking
?(1) 1mg BSA was dissolved in 0.3 mL PBS (solution A), and 0.2 mg NSP-SA-NHS was dissolved in 40 μL DMF (solution B).
?(2) Add solution B to solution A, mix at 18°C, 130 rpm, and dark for 12 h.
?(3) The mixture was dialyzed with 500 mL of distilled water at 4°C for 2 d, during which distilled water was changed twice (solution C).
?(4) Slowly add solution B to solution C, mix at 18°C, 130 rpm, and dark for 24 h, and then dialysis with distilled water for 48 h. During this period, change distilled water four times.
?(5) Dilute the coupling compound with distilled water to a suitable concentration, and analyze it with an ultraviolet spectrophotometer.

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