重组人DHH C23II北京厂家

特别提示：包括重组人DHH C23II在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组人DHH C23II
英文名称：Recombinant Human Desert Hedgehog Cys23IleIle
产品货号：GS4745
产品规格：5μg

分子量：Approximately 19.9kDa,a single non-glycosylated polypeptide chain containing 177 amino acids.
储存条件：冷冻（-20℃）

概述

Desert Hedgehog(Dhh)belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes.Desert Hedgehog is a secreted,45 kDa,373 amino acid protein that undergoes autocatalytic cleavage between Gly198 and Cys199 catalyzed by the C-terminal domain,which releases the N-terminal domain with a concominant attachment of cholesterol at its new C-terminus.Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein(Hip).Within the N-terminal domain,human Dhh shares 97%aa sequence identity with mouse and rat Dhh.It shares approximately 75%aa seqeuence identity with human Indian(Ihh)and Sonic Hedgehog(Shh).Dhh is expressed in the female by ovarian granulosa cells and the corporus luteum.Its upregulation in ovarian cancer correlates positively with proliferative index,and negatively with prognosis.Dhh is also expressed by Schwann cells and is upregulated following nerve injury.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞96%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226)cells is 15-45μg/ml.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in 10mMPB,pH 6.0,300mM NaCl.
内毒素	Less than 1 EU/μg of rHuDHH C23II as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

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