InSolution Y-27632 in DMSO - C

产品描述 General description A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688001) in H2O and the solid form of this compound (Cat. No. 688000) are also available. A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. Other Notes Watanabe, K., et al. 2007. Nature Biotech.25, 681. Chitaley, K., et al. 2001. Nat. Med.7, 119. Davies, S.P., et al. 2000. Biochem. J. 351, 95. Narumiya, S., et al. 2000. Methods Enzymol.325, 273. Hirose, M., et al. 1998. J. Cell Biol.141, 1625. Maekawa, M., et al. 1999. Science285, 895. Uehata, M., et al. 1997. Nature389, 990.