Biofroxx 德国 Agarose 琼脂糖 1110GR

Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period

100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high

PCR基因扩增技术

酶。[实验仪器与设备]1. PCR基因扩增仪2. 琼脂糖凝胶电泳系统[实验材料]1. DNA模板2. 4种dNTP3. 引物1和引物24. Taq酶5. 琼脂糖6. DNA Marker7. tip8. eppendorf管9. 微量移液器附试剂的配制：1. 10×PCR缓冲液500 mmol/L KCl100 mmol/L Tris·HCl(Ph8.3,室温)15 mmol/L MgCl20.1% 明胶2. 4×dNTP10 mmol/L dATP10 mmol/L dCTP

GST pull down

: RXR without DNA, your wash buffer must be 1x PBS supplemented with 500mm NaCl. Material and solutions: IPAB-gelatin: HEPES 20mM pH7.8 KCl 150mM Gelatin 0.1% TritonX100 0.1% NP40 0.1% MgCl2 5mM DTT 2mM Wash buffer: PBS 1X DTT 2mM TnT Quick T