- ￥2850
- epitomics
- 2207-1
- USA
- 2025年07月24日
- 免疫细胞化学
- 兔
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Anti-Btk Phospho (pY223) (BTK)，BTK,AGMX1, AT, ATK, BPK
- 应用范围：
免疫细胞化学
- 宿主：
兔
- 库存：
大量
- 供应商：
上海研卉生物
- 适应物种：
人
- 抗原来源：
其它
- 是否单克隆：
0
- 规格：
100ul
| 【产品类型】Rabbit Monoclonal Antibody | |
| 【产品批号】 | 【价格】2850元 |
| 【反应特异性】Human , Mouse , Rat | 【克隆 ID】EP420Y |
| 【用途】WB , IP | 【产品性状】 |
| 【阳性对照】 | 【产品浓度】 |
| 【细胞定位】 | 【抗原分子量】77 kDa |
| 【Database links】Swiss Prot: Q06187 | |
| 【其它名称】BTK,AGMX1, AT, ATK, BPK, IMD1, MGC126261, MGC126262, PSCTK1, XLA,Tyrosine-protein kinase BTK,Bruton tyrosine kinase | |
【产品介绍】Btk (Bruton tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. Btk is composed of a pleckstrin domain, an SH3 and SH2 domain, a catalytic kinase domain and a TEC homology domain (1). Tec family protein tyrosine kinases (TFKs) play a central role in hematopoietic cellular signaling. Initial activation takes place through specific tyrosine phosphorylation situated in the activation loop. Further activation occurs within the SH3 domain via a transphosphorylation mechanism, which for Bruton's tyrosine kinase (Btk) affects tyrosine 223. (2). Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner (3). Btk can be activated through intramolecular autophosphorylation at tyrosine 551 and that Btk autophosphorylation is required for Btk catalytic functions (4).
|
Recommended ApplicationsWB, IP Applications and Recommended Dilution Factors WB: 1:10,000 - 50,000 Species Reactivity *Human, Mouse, Rat *Mouse and Rat reactivity predicted based on sequence alignment only, not based on testing
Products Data
A. Western blot analysis on Ramos cell lysates using anti-Phospho-BTK (pY223) RabMAb (cat. #2207-1). Cells were either (A) untreated (B) treated with pervanadate.
SpecificityA phospho specific peptide corresponding to residues surrounding tyrosine 223 of human BTK was used as an immunogen. This antibody detects BTK phosphorylated on tyrosine 223. Storage Condition and BufferStore at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
1. Tsukada, S., et al (1993) Cell 72, 279-290
2. Rawlings, DJ et al. Immunol. 7, 237-246
3. Vassilev, AZ et al.. 1999. J. Biol. Chem. 274:1646-1656
4. Mahajan S, et al. Mol Cell Biol. 1995;15:5304
Order: 877-772-2622
Support: support@epitomics.com
Web: www.epitomics.com
| Quantity: 100ul | Lot #: Please refer to vial |
| Clone ID: EP420Y | Molecular Wt.: 77 kDa |
| Species Cross-reactivity* +Human+Mouse+Rat | UniProt ID: Q06187 |
| Applications: +WB-IHC-ICC+IP-FC |
Background: Btk (Bruton tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. Btk is composed of a pleckstrin domain, an SH3 and SH2 domain, a catalytic kinase domain and a TEC homology domain (1). Tec family protein tyrosine kinases (TFKs) play a central role in hematopoietic cellular signaling. Initial activation takes place through specific tyrosine phosphorylation situated in the activation loop. Further activation occurs within the SH3 domain via a transphosphorylation mechanism, which for Bruton's tyrosine kinase (Btk) affects tyrosine 223. (2). Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner (3). Btk can be activated through intramolecular autophosphorylation at tyrosine 551 and that Btk autophosphorylation is required for Btk catalytic functions (4).
Specificity: A phospho specific peptide corresponding to residues surrounding tyrosine 223 of human BTK was used as an immunogen. This antibody detects BTK phosphorylated on tyrosine 223.
Storage Condition and Buffer: Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Recommended Dilutions:
WB: 1:10,000 - 50,000
IP: 1:40
Background References:
1. Tsukada, S., et al (1993) Cell 72, 279-290
2. Rawlings, DJ et al. Immunol. 7, 237-246
3. Vassilev, AZ et al.. 1999. J. Biol. Chem. 274:1646-1656
4. Mahajan S, et al. Mol Cell Biol. 1995;15:5304
*Mouse and Rat reactivity predicted based on sequence alignment only, not based on testing
| Fig 1. A. Western blot analysis on Ramos cell lysates using anti-Phospho-BTK (pY223) RabMAb (cat. #2207-1). Cells were either (A) untreated (B) treated with pervanadate. |
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文献和实验Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation
The ERK signaling cascade is composed of several protein kinases that sequentially activate each other by phosphorylation. This pathway is a central component of a complex signaling network that regulates important cellular processes
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Figure 18.20.1 The specificity of phospho‐motif antibodies. HeLa cells were serum‐starved for 24 hr prior to being stimulated with 50 ng/ml IGF‐1 or 100 nM PMA for 10 min. Lysates were immunoblotted with anti‐Akt/PKB phospho‐motif (left panel
是B细胞发育受阻不能有效地产生抗体。 究其原因，是因XLA患者X染色体长臂(q21-22．3区带)上一个基因发生了突变，该基因的编码产物为胞质中的酪氨酸激酶Btk，全称为Bruton酪氨酸激酶(Bruton tyrosine kinase)，于Tec激酶家族。btk基因的突变方式可以为点突变，也可以为DNA片段缺失。 Btk不仅参与成熟B细胞抗原识别信号的转导，并在前B细胞信号转导中起关键作用。B细胞从原B向前B分化后，出现一种称为前B细胞受体(pre-BCR)的结构
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