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- 397902
- 2025年10月02日
- Flow Cytometry/FC
- 小鼠
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体名：
CD133/PROM1
- 抗体英文名：
Purified anti-human CD133 Antibody
- 浓度：
0.5 mg/mL
- 应用范围：
Flow Cytometry/FC
- 宿主：
小鼠
- 适应物种：
人
- 目录编号：
397902
- 标记物：
无
- 是否单克隆：
单克隆
- 保存条件：
低温
- 亚型：
Purified Mouse IgG1, κ Isotype Ctrl
- 规格：
100ug
货号：397902
【产品介绍】CD133是120kDa糖基化 蛋白，包括5个跨膜结构域。1997年Yin等首次报道了AC133抗原并成功制备了能识别该抗原的单克隆抗体，2000年6月第七届人类白细胞分化抗原 工作组会议(ILDAW)将此抗原命名为CD133。文献报道：CD133+CD34+的造血干/祖细胞较之CD133-CD34+造血干/祖细胞有更强 的多系分化潜能，从而为白血病的造血干细胞移植提供新的策略。近来经研究发现CD133分子是HSCs（造血干细胞）特异性标志。CD133即 AC133，是一个新发现的HSCs（造血干细胞）表面标志，在HSCs（造血干细胞）分化成熟过程中，CD133的含量迅速降低。EPCs(血管内皮前 体细胞)区别于成熟内皮细胞的主要标志是CD133。
描述
CD133，也称为Prominin-1和AC133抗原，是一种具有5个跨膜结构域的120kD五跨糖蛋白。CD133最初被描述为人类造血干细胞特异性的表面抗原，以及小鼠神经上皮细胞和一些胚胎上皮细胞的标志物。后来，在其他干细胞上发现了CD133，包括内皮祖细胞、胶质母细胞瘤、神经元和神经胶质干细胞。除了正常组织的干细胞外，在癌症细胞上还发现了CD133，如一些白血病细胞和脑肿瘤细胞。尽管CD133的生物学功能尚不完全清楚，但CD133已被广泛用作正常和癌组织的干细胞标志物。
- Clone
- W6B3C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prominin-1, PROM1, AC133
- Isotype
-
Mouse IgG1, κ
Pricing & Availability
- Clone
- W6B3C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prominin-1, PROM1, AC133
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- 1 publications
NCCIT cells were stained with purified anti-human CD133 (clone W3B3C1) (filled histogram) or Mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
Compare all formatsCat # Size Price Quantity Check Availability Save 397902 100 µg $273 DescriptionCD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
- Isotype Control
- Purified Mouse IgG1, κ Isotype Ctrl
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- WERI-RB-1 human retinoblastoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
W6B3C1 antibody does not compete against AC133, 293C2, clone 7, S16015F, S16016B, or S16016E antibody binding to target.
-
Application References
(PubMed link indicates BioLegend citation) -
- Hermansen SK, et al. 2011. J. Histochem Cytochem. 59(4): 391.
- Rappa G, et al. 2008. Stem Cells. 26(12): 3008.
- Tardito S, et al. 2015. Nat Cell Biol. 17(12): 1556.
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- 作者
- 内容
- 询问日期
文献和实验- (PubMed link indicates BioLegend citation)
-
- Hermansen SK, et al. 2011. J. Histochem Cytochem. 59(4): 391.
- Rappa G, et al. 2008. Stem Cells. 26(12): 3008.
- Tardito S, et al. 2015. Nat Cell Biol. 17(12): 1556.
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