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- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
上海研卉生物
- 规格:
100ml
CryoStem™ hPSC Freezing Medium 无血清冻存液
Product Overview:
CryoStem™ hPSC Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Cells preserved with CryoStem™ hPSC Freezing Medium show high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem™ hPSC Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem™ hPSC Freezing Medium has been tested on human ES cells (H1, H9 and HuES9). Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products.
CryoStem™ hPSC Freezing Medium contains methylcellulose and DMSO in place of serum. The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived products.
CryoStem™ hPSC Freezing Medium Features:
Chemically defined, Animal component-free (ACF), Protein-free
Works with various media
Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
High recovery efficiency: maintains excellent attachment ability as well as growth performance
Maintains hESC and hiPSC pluripotency
Complete formulation; Ready-to-use at 2-8°C
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文献和实验的主要特点: 1、适用于骨髓/脐带/脂肪来源的MSC 2、在更短的培养时间内细胞扩增更多 3、细胞倍增速度更快 4、作简便,基础培养补充剂一次性混合后,即可实现培养 5、更低的接种密度,4000个细胞/cm2 6、在细胞传代的整个过程中,都可持免疫表型、多能性及对T细胞的抑制能 7、无血清、无动物源性,成分已知 优点: 1、精确
当前,多种细胞疗法已应用于临床,另外还有更多的细胞疗法在进行着临床试验,但是,不同的细胞疗法也产生了不同的临床结果。比如说,造血干细胞、嵌合抗原受体 T 细胞(CAR-T)的治疗已经成功挽救数以万计的患者,但仍有相当大比例的细胞治疗方案仅有微弱的临床效果,特别是间充质干细胞 (MSCs),其在过去 20 年里一直是一个高度关注的课题。据不完全统计,MSCs 治疗在一系列疾病中有超过 700 个临床试验报告。总的来说,间充质干细胞具有积极的安全性,但大多数间充质干细胞移植治疗的有效性仍未实现
培养细胞。 19、干细胞在更换外泌体专用间充质干细胞培养基的过程中,遇到干细胞生长缓慢,甚至死亡的现象,该如何处理? 答:干细胞在更换培养基的过程中,对于培养环境比较敏感,尤其是从高营养环境(血清/HPL添加培养基)更换到低营养环境(化学成分确定培养基)的过程中会遇到干细胞生长缓慢,甚至死亡的现象,这些都是更换培养体系过程中经常遇到的问题。 解决方案 1:提高更换培养基时干细胞的融合度。细胞融合度从说明书中 20% 提高到50%-60% 时,然后更换外泌体专用间充质干细胞培养基,然后在细胞融合度 70%-80
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