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05-712-1B/05-712-1E
| 规格： | 05-712-1B | 产品价格： | ¥960.0 |
|---|---|---|---|
| 规格： | 05-712-1E | 产品价格： | ¥640.0 |
货号：05-712-1B/E
规格：50ml，100ml
产品名称：CryoStem™ hPSC Freezing Medium 无血清冻存液
Product Overview：
CryoStem™ hPSC Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Cells preserved with CryoStem™ hPSC Freezing Medium show high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem™ hPSC Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem™ hPSC Freezing Medium has been tested on human ES cells (H1, H9 and HuES9). Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products.
CryoStem™ hPSC Freezing Medium contains methylcellulose and DMSO in place of serum. The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived products.
CryoStem™ hPSC Freezing Medium Features：
Chemically defined, Animal component-free (ACF), Protein-free
Works with various media
Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
High recovery efficiency: maintains excellent attachment ability as well as growth performance
Maintains hESC and hiPSC pluripotency
Complete formulation; Ready-to-use at 2-8°C
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文献和实验, this method for preparing competent E. coli from Inoue et al. (1990) can challenge the efficiencies achieved by Hanahan (1983). However, under standard laboratory conditions, efficiencies of 1 x 108 to 3 x 108 transformed colonies/mg of plasmid DNA
Hints and precautions for the care, feeding and breeding of Neurospora
ascospores showing the segregation of two genes in a cross of col-4 x pyr-2. Spores were isolated on minimal agar medium in petri dishes, heated at 60° for 30 minutes for activation, and incubated at 25° for 18 hours. This nutritional mutant grows
Maintenance/Metabolism Working Medium pre-warmed into a 92 x 17 mm Petri dish. e. Remove the lid from the multi-well plate. f. Remove the existing medium from the wells. g. Gently add the pre-warmed HepaRG™ Maintenance
