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- 2025年08月14日
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1 kit
Angiogenesis Tube Formation Assays
In Vitro Angiogenesis cell based assays provide a convenient system for the evaluation of endothelial cell tube formation in a convenient 96-well format using either ECM Gel or Fibrin ECM gels.
| Product No. | Product Description |
|---|---|
| ECM625 | In Vitro Angiogenesis Assay Kit |
| ECM630 | Fibrin In Vitro Angiogenesis Assay |
| E1270 | ECM Gel from Engelbreth-Holm-Swarm murine sarcoma |
| E6909 | ECM Gel (Growth Factor Reduced) |
| F5386 | Fibrin from Human Plasma |
Figure 2. HUVEC Angiogenesis Assay. HUVEC cells form extensive tubular vascular networks after incubation of 6-10 hours at 37°C on ECM Matrix provided in the In Vitro Angiogenesis Assay Kit (Product No. ECM625).
Microfluidic Angiogenesis Assays
Studying how compounds affect angiogenesis, either to promote or inhibit new capillary tube formation can lead to therapies affecting wound healing, tissue regeneration, cardiovascular disease, stroke, tumor progression, and more. The Millicell μ-Angiogenesis activation and inhibition kits provide a powerful, quantitative platform for live cell monitoring of changes in tubule formation with unprecedented optical resolution.
| Product No. | Product Description |
|---|---|
| MMA125 | Millicell® µ-Angiogenesis Inhibition Assay Kit |
| MMA130 | Millicell® µ-Angiogenesis Activation Assay Kit |
Figure 3. Increasing concentrations of sulforaphane resulted in decreases in both mean tube length and mean number of branch points as shown below in bright field and calcein-AM micrographs of HUVEC cells.
Endothelial Adhesion, Invasion and Migration Assays
Endothelial cells invade through the basement membrane to form sprouting blood vessels within tumors. The invasion process consists of the secretion of matrix metalloproteases (MMPs) to degrade basement membrane, the activation of endothelial cells, and the migration of cells across the basement membrane6. The understanding of invasion is important for studying the mechanism of angiogenesis in injured tissue as well as in disease such as cancer.
QCM™ Boyden chamber cell invasion assays enable convenient and sensitive quantification of in vitro cell invasion, vascular permeability, adhesion and migration through an endothelial cell layer.
| Product No. | Product Description |
|---|---|
| ECM200 | QCM 3 µm Endothelial Cell Migration Assay Fibronectin, Colorimetric |
| ECM201 | QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric |
| ECM210 | QCM Endothelial Cell Invasion Assay (24 well, Colorimetric) |
| ECM211 | QCM Endothelial Cell Invasion Assay (24 well, Fluorometric) |
| ECM557 | QCM Leukocyte Transendothelial Migration Assay (Colorimetric, 24 Assays) |
| ECM558 | QCM Tumor Cell Transendothelial Migration Assay (Colorimetric, 24 Assays) |
| ECM642 | In Vitro Vascular Permeability Assay (96-well) |
| ECM644 | In Vitro Vascular Permeability Assay (24-well) |
| ECM645 | Endothelial Cell Adhesion Assay Kit |
Scratch Wound Healing Assays
The scratch assay is a popular method for the study of cell migration and angiogenesis7. Briefly, endothelial cells are grown to confluence and a wound is introduced by clearing an area of the monolayer using a pipet tip, needle or cell scraper. Cell filling of the cleared space initially occurs by migration, though cells in the cleared area will eventually also proliferate. Scaling up this technique has proved challenging, however, making biochemical analysis of the molecular events mediating wound repair difficult. The Cell Comb™ scratch assay addresses the need for a simple tool able to create multiple scratch wounds in a higher-throughput manner.
Read our Application Note in Nature
| Product No. | Product Description |
|---|---|
| 17-10191 | Cell Comb™ Scratch Assay |
Endothelial Cell Characterization
| Product No. | Product Description |
|---|---|
| ECM590 | Blood Vessel Staining Kit |
| SCR023 | Endothelial Cell Characterization Kit |
| HPA001815 | Anti-VWF antibody produced in rabbit |
| V9263 | Monoclonal Anti-Vascular Cell Adhesion Molecule 1 antibody |
| V7259 | Vascular Endothelial Growth Factor human (VEGF) |
Endothelial Cells and Culture Media
Primary cells maintain physiological relevance because they are derived directly from living tissue and thus find increasing use in life science research and pharmaceutical drug. We are pleased to offer primary cells from Cell Applications Inc. including endothelial cells and optimized growth media from microvascular, aortic, coronary, pulmonary, lung and umbilical cord (HUVECs) tissues.
Endothelial Cell Culture Media
Video: Introduction to Primary Cell Culture
Figure 4. (A) Cobblestone morphology of Human Umbilical Vein Endothelial Cells, HUVEC; (B) HUVEC immunolabeled with VEGFR2 antibodies (green); (C) HUVEC stained with DiI-Ac-LDL (red), the acetylated apoprotein specifically recognized and endocytosed by endothelial cells; (D) HUVEC form vessel-like CD31/PECAM positive (green) structures when cultured with Human Dermal Fibroblasts in the presence of VEGF.
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。 5.如果没有其他参数修改或输入，光标20秒后将自动熄灭。 6.以同样的方法改变其它参数。 三、注意事项 1.离心机应放在平稳且通风良好的台面上。 2.仪器周围至少有30厘米的空间。 3.离心时一定要配平，放置要对称。 4.离心前一定要检查安装的转头编号与离心机程序所设定转头编号是否一致。 5.每次使用完以后必须将盖子打开让离心腔回到室温，并且用软布将离心腔里冷却水和赃物清理干净。 6.转头转轴和螺丝要经常上油（SIGMA货号NO70284），吊篮和适配器经常清洗上油（SIGMA 货号
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