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文献和实验Comprehensive High‐Throughput Arrays for Relative Methylation (CHARM)
) 100 mM (100×) guanosine triphosphate (GTP; store in single‐use aliquots at −80°C) 10 U/µL McrBC (New England Biolabs): test each new lot on a standard gDNA sample
Targeted Exon Sequencing by In‐Solution Hybrid Selection
Master Mix (Stratagene, cat. no. 600548) 1 mM ROX Reference Dye 1.25 µM P5 PCR primer (AATGATACGGCGACCACCGA
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
al 1992, Vaysse and Goldman, 1990). The indigo product of X-gal absorbs in the wavelengths emitted by the standard fluorescent-conjugated antibodies and is so dark that it can obscure the products from either horseradish peroxidase or alkaline
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